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oVERVIEWPATIENT
CARERESEARCHEDUCATIONFACULTYCONTACTSHOME |
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Faculty Nathan Wolins, Ph.D. Research Interests Animals are dependent on feeding for energy in a world with limited feeding opportunities. To secure the energy and retain the mobility to find their next meal, animals convert the food into energy-dense fat droplets. Our behavior and fat storage mechanisms are adapted to survive food scarcity. Specifically, we are driven to eat large amounts of high energy food and store the excess as fat. Overeating has caused rampant obesity. Obesity is associated with excess circulation of nutrients, lipid accumulation in nonadipose tissue, insulin resistance, arteriosclerosis and cardiomyopathy. Our work focuses on a set of proteins that move from the cytosol to the fat/cytosol interface during feeding induced fat storage. When the nutrient bolus is cleared and fat accumulation slows, these proteins move from the fat/cytosol interface back into the cytosol. We hypothesize that these proteins, in part, determine the intracellular fat storage capacity and resistance to overeating driven disease. Contact Information Nathan Wolins, Ph.D. Publications Recent Articles in Peer Review Journals 1. Schwartz D..M. and Wolins N.E. (2007). A simple and rapid method to assay triacylglycerol in cells and tissues. J Lipid Res 48, 2514-20 Wolins N.E., Quaynor B.K., Skinner J.R., Tzekov A., Croce M.A., Gropler M.C., Varma V., Yao-Borengasser A., Rasouli N., Kern P.A., Finck B.N., and Bickel P.E. (2006). OXPAT/PAT-1 Is a PPAR-Induced Lipid Droplet Protein That Promotes Fatty Acid Utilization. Diabetes 55, 3418-28. 3. Wolins N.E., Brasaemle D.L., and Bickel PE (2006) A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 580, 5484-91. Wolins, N. E., Quaynor, B. K., Skinner, J. R., Tzekov, A., Park, C., Choi, K., and Bickel, P. E. (2006). OP9 mouse stromal cells rapidly differentiate into adipocytes: characterization of a useful new model of adipogenesis. J Lipid Res 47, 450-460. Wolins, N. E., Quaynor, B. K., Skinner, J. R., Schoenfish, M. J., Tzekov, A., and Bickel, P. E. (2005). S3-12, Adipophilin, and TIP47 package lipid in adipocytes. J Biol Chem 280, 19146-19155. 6. Brasaemle D.L., and Wolins, N. E. (2005). Isolation of lipid droplets from cells by density gradient centrifugation. Current Protocols in Cell Biology, Unit 3.15, John Wiley & Sons, Inc., New York. Wolins, N. E., Skinner, J. R., Schoenfish, M. J., Tzekov, A., Bensch, K. G., and Bickel, P. E. (2003). Adipocyte protein S3-12 coats nascent lipid droplets. J Biol Chem 278, 37713-21. Wolins, N. E., Rubin, B., and Brasaemle,
D. L. (2001). TIP47 associates with lipid droplets. J Biol Chem 276, 5101-8.
Department of Medicine
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